Comparative Studies on the Interaction of Human and Bovine Serum Albumins with Vitamin C
Date
2018-05-11Author
Alsamamra, Husain
Abusharkh, Sawsan
Abuteir, Musa
Metadata
Show full item recordAbstract
The interactions of human and bovine serum albumins (HSA and BSA) with various drugs using spectroscopic
techniques have received great attention now a days due to their significant effect in the biomedical field. UV absorption and
fluorescence spectroscopy are the most likable due to its high sensitivity and simplicity. The interaction of HSA and BSA with
vitamin C was investigated. Results showed that the absorption and fluorescence intensities increased as the vitamin C
concentration increases. The calculated binding constant (k ~104 M-1) showed a week binding of vitamin C with both serum
albumins. The analysis of fluorescence quenching for HSA/BSA-vitamin C interaction (kq ~ 1011 L mol-1 s-1) reveals the
dynamic quenching process and clearly confirms the existence of static mechanism of fluorescence quenching.