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dc.contributor.authorAbdul-Ghani, Safa
dc.contributor.authorFleishman, Arnold N.
dc.contributor.authorKhaliulin, Igor
dc.contributor.authorMeloni, Marco
dc.contributor.authorAngelini, Gianni D.
dc.contributor.authorSuleiman, M-Saadeh
dc.date.accessioned2019-03-26T14:02:43Z
dc.date.available2019-03-26T14:02:43Z
dc.date.issued2017-02-05
dc.identifier.issn2051-817X
dc.identifier.urihttps://dspace.alquds.edu/handle/20.500.12213/4761
dc.description.abstractCardioprotective efficacy of remote ischemic preconditioning (RIPC) remains controversial. Experimental studies investigating RIPC have largely monitored cardiovascular changes during index ischemia and reperfusion with little work investigating changes during RIPC application. This work aims to identify cardiovascular changes associated with autonomic nervous system (ANS) activity during RIPC and prior to index ischemia. RIPC was induced in anesthetized male C57/Bl6 mice by four cycles of 5 min of hindlimb ischemia using inflated cuff (200 mmHg) followed by 5 min reperfusion. Electrocardiography (ECG) and microcirculatory blood flow in both hindlimbs were recorded throughout RIPC protocol. Heart rate variability (HRV) analysis was performed using ECG data. Hearts extracted at the end of RIPC protocol were used either for measurement of myocardial metabolites using high-performance liquid chromatography or for Langendorff perfusion to monitor function and injury during 30 min index ischemia and 2 h reperfusion. Isolatedperfused hearts from RIPC animals had significantly less infarct size after index ischemia and reperfusion (34 5% vs. 59 7%; mean SE P < 0.05). RIPC protocol was associated with increased heart rate measured both in ex vivo and in vivo. Frequency ratio of HRV spectra was altered in RIPC compared to control. RIPC was associated with a standard hyperemic response in the cuffed-limb but there was a sustained reduction in blood flow in the uncuffed contralateral limb. RIPC hearts (prior to index ischemia) had significantly lower phosphorylation potential and energy charge compared to the control group. In conclusion, RIPC is associated with changes in ANS activity (heart rate, blood flow, HRV) and mild myocardial ischemic stress that would contribute to cardioprotection. Introduction Remote ischemic preconditioning (RIPC) is a phenomenon where preconditioningen_US
dc.description.sponsorshipThis work was funded by the British Heart Foundation (PG/15/66/31710) and the National Institute for Health Research Bristol Biomedical Research Unit in Cardiovascular Disease.en_US
dc.language.isoenen_US
dc.publisherWiley-Blackwell on behalf of The Physiological Society and the American Physiological Societyen_US
dc.subjectAutonomic nervous systemen_US
dc.subjectblood flowen_US
dc.subjectcardiacen_US
dc.subjectischemiaen_US
dc.subjectmote ischemic preconditioningen_US
dc.titleRemote ischemic preconditioning triggers changes in autonomic nervous system activity: implications for cardioprotectionen_US
dc.typeArticleen_US


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