Investigating The Regulation of MIR503HG and POT1-AS1 Expression in Breast Cancer Cells by Microenvironmental Cues: Hypoxia, Oxidative Stress, Serum Starvation, and HGF
Date
2025-06-01
Authors
Aseel Raqaban
Journal Title
Journal ISSN
Volume Title
Publisher
Deanship of Research - Al-Quds University
Abstract
Long non-coding RNAs (lncRNAs) are increasingly recognized as key regulators within the tumor microenvironment, particularly in how cancer cells adapt to stressful conditions such as hypoxia, starvation, and oxidative stress through transcriptional reprogramming. However, the expression patterns of many lncRNAs, including MIR503HG isoform 2 and POT1-AS1, remain poorly understood under these conditions. This study aimed to investigate the expression of these two lncRNAs in MDA-MB-231 triple-negative breast cancer (TNBC) cells exposed to serum starvation, H2O2-induced oxidative stress, hepatocyte growth factor (HGF) stimulation, and hypoxia. A semi-quantitative PCR approach was used, followed by gel electrophoresis and densitometric analysis using ImageJ software to quantify band intensities.