OPTIMIZING MAGNESIUM QUANTIFICATION IN HUMAN SERUM: A COMPARATIVE EVALUATION OF XYLIDYL BLUE-BASED REAGENTS
Date
2026-05-12
Authors
Hiba Jafar Al-Halabieh
Jumana Khalid Odeh
Yumna Bassam Afaneh
Alaa Drabee
Journal Title
Journal ISSN
Volume Title
Publisher
Deanship of Scientific Research - Al-Quds University
Abstract
Background: Magnesium (Mg2+) is a vital mineral essential for numerous physiological and biochemical processes. Accurate quantification of serum magnesium is clinicaly imperative for diagnosing electrolyte imbalances and managing chronic health conditions. While the Xylidyl Blue method remains a standard technique for magnesium measurement, its reliability is highly dependent on reagent composition and specific analytical conditions. This study aimed to optimize this methodology by evaluating various reagent formulations to enhance diagnostic accuracy.
Methods: A systematic assessment was conducted on twelve distinct reagent formulations to determine their efficacy in measuring magnesium concentrations in human serum. Each formulation was rigorously evaluated for accuracy, precision, stability, and sensitivity using a standardized analytical protocol. Following initial screening, the optimal formulation, designated as Reagent R7, was selected for comprehensive validation. Its performance was benchmarked against a recognized commercial magnesium quantification kit (ELITech) using Bland-Altman analysis to assess clinical agreement.