Searching for potential novel Orf virus epitopes using reverse vaccinology

Simple item page
dc.contributor.authorAL-Shareef, Aseel
dc.contributor.authorAl-Natsheh, Raghad
dc.contributor.authorAbu Omar, Zainab
dc.contributor.authorAbu Ghazaleh, Robin
dc.date.accessioned2021-01-02T10:19:08Z
dc.date.available2021-01-02T10:19:08Z
dc.date.issued2020-12-22
dc.description.abstractOrf virus is a zoonotic virus that mainly affects sheep and goats and causes skin lesions, which reduce the feeding process among their lambs and kids. An Orf virus vaccine is available, however, the immunity it induces doesn’t last for more than one year, making the reinfection of the virus very common. This research aims to find epitopes that could be a good target for a long-term protein-based vaccine. Using reverse vaccinology, all proteins of the three Orf virus strains (ORFV-SA00, ORFV-NZ2 & ORFV-SY17) were studied by searching for proteins that could have good subcellular localization, antigenicity, as well as being conserved among the three genomes. After selecting proteins with these properties, linear B-cell and T-cell epitopes were detected. The last step was to test the stability of these chosen epitopes by searching for potential proteasomal cleavage sites. This final step in the bioinformatics discovery pipeline left a single stable epitope candidate (DRRPCGVQD). This protein (epitope) is recommended to be tested experimentally to ensure its effectiveness as a vaccine target protein.en_US
dc.identifier.urihttps://dspace.alquds.edu/handle/20.500.12213/6298
dc.language.isoenen_US
dc.publisherAl-Quds University, Deanship of Scientific Researchen_US
dc.titleSearching for potential novel Orf virus epitopes using reverse vaccinologyen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Searching for potential novel Orf virus epitopes using reverse vaccinology.pdf
Size:
289.5 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.61 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Natural sciences