Natural sciences

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Browsing Natural sciences by Author "Ishnaiwer, Murad"

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    Detection of Virulence Gene Profiles of Multi-Drug Resistance (MDR) in Pseudomonas aeruginosa Bacteria in Human
    (Al-Quds University, Deanship of Scientific Research, 2020-12-22) Abu-Fanar, Aysha; Al-Jabary, Bisan; Hrinat, Maalem; Ishnaiwer, Murad; Al-Razem, Fawzi
    The spread of opportunistic bacteria Pseudomonas aeruginosa in hospitals correlated with several diseases to humans. This bacterium showed high resistance to many antibiotics due to their indiscriminate use, as well as mutations in the pathogenic genes of Pseudomonas aeruginosa, which are difficult to treat. Pseudomonas aeruginosa is transmitted to humans in hospitals as being special nosocomial bacteria through ventilators, also through food and water contaminated with these bacteria, which causes muscle fatigue, vomiting and nausea. It also weakens the immune system and is more severe in people who suffer from weak immunity, old age and children, and can lead to death. Therefore, scanning virulence genes in Pseudomonas aeruginosa helps understand pathogenesis mechanisms. The main objective of this study was to detect three pathogenic genes in these bacteria: ExoS, Apr, and Pich. Bacteriological samples were collected and detected by the Multiplex PCR mechanism. Results showed that the sizes of genes were: 444bp, 1017bp, and 608bp. The study of the reaction of multiplex polymers to detect genes (ExoS, Apr, Pich) in 32 colonies was provided by Al-Ahli Hospital in Hebron, the percentage of each gene was 95%. In addition to 12 samples provided by the Al-Istishari Hospital in the city of Ramallah, and the percentage of the presence of each gene in these samples 100%. In the antibiotic examination of Pseudomonas aeruginosa of 44 samples, the bacteria had antibiotic resistance, 43% and 31% of the bacterial strains were resistant to Gentamicin and Aztreonam, 27% of which were resistant to Meropenem, Ceftazidime and Ciprofloxacin, 18% of which were resistant to tazobactam and 22.7% of which were resistant to Amikacin. The minor objective was to identify MDR Pseudomonas aeruginosa from clinical isolates. Isolates were evaluated for their antimicrobial susceptibility to seven antibiotics, Meropenem, Ceftazidime, Amikacin, Azteronam, Ciprofloxacin, Gentamicin and Piperacillin-tazobactam. The results also provided a clear picture of bacterial resistance MDR to these antibiotics by 45.45%.
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    Isolation of bacteria that are able to digest keratin as an alternative to harmful chemicals used in tanning industries
    (Al-Quds University, Deanship of Scientific Research, 2020-12-22) Bheis, Dalia; Abu Sbaih, Naheda; Najjar, Nedaa; Harahshe, Ienas; Al-Razem, Fawzi; Ishnaiwer, Murad
    Abstract:Macroccocus is a bacteria that was isolated from a soil sample containing decaying hair and It was found to contain keratinase enzyme due to its high ability to digest keratin protein. The isolated bacteria by this study can be used as an alternative to harmful chemicals tanning processes represents a safe and, clean technology that achieves economic feasibility in the process of skin soaking and hair removal. Background: Leather tanning is considered one of the ancient professions that Hebron has been known for hundreds of years which is the only source of raw materials for Palestinian factories thatdepends on this commodity. In the past, the industry relied on environmentally friendly natural materials in tanning, but today they have become a source of pollution of soil and groundwater, especially after the entry of chemicals in this craft, such as chromium and some acids that infect humans and destroy the Palestinian environment because of the toxic gases that result from the process of soaking and hair removal. Objectives: 1. To Isolate keratinolytic bacteria from soil sample 2. To test the bacterial effectiveness for hair degradation 3. To identify and characterize of bacteria by sequencing tool. Methods: Soil sample containing decaying hair was collected from the industrial area in Hebron city and processed in the lab, bacteria were isolated by using spreading and streaking plate technique, their ability for hair degradation was tested by incubating bacterial colony, hair and water for several days, after the bacterial ability for hair degradation appeared bacterial isolate was identified by sequencing tool and the type of bacteria was determined by bioinformatics tool. Results: Isolated a bacterial strain that is able to digest keratin and was tested on several samples and proved to be effective in degrading hair. It has been welcomed by the Modern Leather Tanning Company (MLTC-Hebron), which has cooperated with us in this project. The strain was genotyped and appeared to belong to macroccocus, this is the first report to find an industrial value for such bacteria which is also safe for human and the environment. Conclusions: Among microbial isolate screened for keratin digestion by using soil sample, a newly isolated Macroccocusequipercicuswas found with a greater ability for hair degradation, the use of such bacteria as an alternative to harmful chemicals tanning processes represents a safe and, clean technology that achieves economic feasibility in the process of skin soaking and hair removal. but in the same time, the full sequence of these strains of bacteria is unknown and not significantly studied. Therefore, additional researches have to be done for characterization of this type of bacteria