Biophysical Interaction of Propylthiouracil with Human and Bovine Serum Albumins

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dc.contributor.authorAlsamamra, Husain
dc.contributor.authorAbuteir, Musa
dc.contributor.authorDarwish, Saqer
dc.date.accessioned2019-02-02T08:45:03Z
dc.date.available2019-02-02T08:45:03Z
dc.date.issued2019-01-30
dc.description.abstractThe physical interaction of antithyroid drug, propylthiouracil was studied with bovine and human serum albumins through UV absorption and fluorescence spectroscopic techniques. The obtained values of the quenching constants were calculated by the Stern-Volmer equation are in the order of 1012 Lmol-1 s-1 indicating that both serum albumins were quenched by the drug in a static manner. The binding constants of the drug interaction with both HSA and BSA proteins are found to be relatively weak and are in the order of 103 M-1. The tryptophan residues of HSA and BSA are most perturbed by the binding process which was authenticated by the fluorescence spectra of both proteins in the presence of propylthiouracil. The importance behind this study is to clarify the mechanism of the interaction between propylthiouracil with HSA and BSA, as well as providing additional values in order to study drug-protein interaction which may facilitate the study of drug metabolism and transportation.en_US
dc.description.sponsorshipThe authors extend their deepest gratitude towards Dr. Jawad Hasan for his support. This work is supported by the German Research Foundation DFG Grant No. DR228/24-2.en_US
dc.identifier.citationAlsamamra H, Abuteir M, Darwish S (2019) Biophysical Interaction of Propylthiouracil with Human and Bovine Serum Albumins. J Biomedical Sci. Vol.8 No.1:2en_US
dc.identifier.issn2254-609X
dc.identifier.urihttps://dspace.alquds.edu/handle/20.500.12213/4638
dc.language.isoenen_US
dc.publisherBMCen_US
dc.subjectPropylthiouracilen_US
dc.subjectHASen_US
dc.subjectBSAen_US
dc.subjectBinding constanten_US
dc.subjectUV absorptionen_US
dc.subjectFluorescence emissionen_US
dc.subjectStatic quenchingen_US
dc.titleBiophysical Interaction of Propylthiouracil with Human and Bovine Serum Albuminsen_US
dc.typeArticleen_US
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