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dc.contributor.authorAbu-Lafi, Saleh A
dc.contributor.authorAkkawi, Mutaz
dc.contributor.authorAbu-Remeleh, Qassem
dc.contributor.authorJaber, Suhair
dc.contributor.authorQutob, Mutaz
dc.contributor.authorLutgen, Pierre
dc.date.accessioned2020-01-29T19:51:38Z
dc.date.available2020-01-29T19:51:38Z
dc.date.issued2018-01-01
dc.identifier.citationAbu-Lafi S, Akkawi M, Abu-Remeleh Q, Jaber S, Qutob M, et al. (2018) Pure Isolates and Preparative HPLC Fractions or Crude Extract of Inula viscosa: Effect on β-hematin Inhibition in vitro. Pharm Pharmacol Int J 6(1): 00145. DOI: 10.15406/ppij.2018.06.00145en_US
dc.identifier.issn2373-6367
dc.identifier.urihttps://dspace.alquds.edu/handle/20.500.12213/5061
dc.description.abstractThe antimalarial activity of wild Inula viscosa (I. viscosa) plant leaves was investigated. The efficacy of the whole crude extract versus preparative HPLC fractions versus pure isolates were compared by measuring their effect on β-hematin inhibition in-vitro. The preparative HPLC experiments consisted of reversed phase preparative column (22.2mm x 250mm, 10μm) and linear gradient of water, acetonitrile as the mobile phase. Injection volume was 1000μl and the wavelengths range were from 200-450 nm using photodiode array detector (PDA). While fractions (IV, V and VI) showed antimalarial potential in comparison to chloroquine positive control, the rest of the fractions did not show any significant inhibition to the β-hematin formation. The antimalarial results showed that whole crude exact of the plant works better than the preparative fractions or the pure isolates presumably due to synergistic effect. The chemical identity of some of the pure isolates was explored using UHPLC-ESi-MS. Moreover, I. viscosa extract powder stayed stable over several years, while many other products such as Artemisia annua extract or Artemisin Combined Therapy (ACT) drugs rapidly lost their efficiency under tropical storage conditions.en_US
dc.description.sponsorshipNone.en_US
dc.language.isoenen_US
dc.subjectInula viscoseen_US
dc.subjectMalariaen_US
dc.subjectHemozoinen_US
dc.subjectβ-hematinen_US
dc.subjectPreparative HPLCen_US
dc.subjectLC-MSen_US
dc.titlePure Isolates and Preparative HPLC Fractions or Crude Extract of Inula viscosa: Effect on β-hematin Inhibition in vitroen_US
dc.typeArticleen_US


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