دراسة جزيئية عن مرض سرطان الثدي في فلسطين: فحص مسستوى البروتين Activation Induced Cytidine Deaminase (AID)
Date
2016-12-20
Authors
منار محمود مصطفى رحال
Manar Mahmoud Mustafa Rahhal
Journal Title
Journal ISSN
Volume Title
Publisher
AL-Quds University
جامعة القدس
جامعة القدس
Abstract
Breast Cancer is the most common cancer among women worldwide with an estimated
more than one million new cases every year, and it is the leading cancer-related cause of
death among women. In Palestine, breast cancer is the most common malignancy in total
population and the most common adult female malignancy according to the Cancer
Registry centers (CRC) and Palestinian Ministry of Health (MOH).
Activation induced cystidine deaminase (AID) is a member of APOBEC family of
cystidine deaminase which deaminate cytosine in DNA leading to uracil. AID is an
essential enzyme normally expressed in B cells. AID enzyme is necessary in diversifying
the immune repertoire by two main processes; the first one is Class Switch Recombination
(CSR), the second process is Somatic Hypermutation (SHM) which allows the immune
system to adapt its response to any threats during the lifetime of an organism. Aberrant
expression of AID causes genomic instability and induces mutations in multiple genes by
favoring chromosome translocations and point mutations in both B and non-B cells,
thereby stimulating cancer formation. AID expression has been reported in numerous
cancers of non-B-cell origin, including breast, prostate, stomach, liver, and lung cancer.
This study aims to screen the expression of AID protein in tissue sections of breast cancer
in patients from Palestine and to determine the correlation between AID protein expression
and the clinico-pathological data of these patients. To achieve this, paraffin blocks of 69
breast cancer with their clinico-pathological data were collected retrospectively, between
years (2009-2013) from the pathology department of Beit Jala Hospital. The expression
levels of AID protein were examined in these cases by Immunohistochemistry (IHC)analysis.
Our results showed an aberrant AID expression in 26.1% (18 of 69) of the breast cancer
cases. No statistical correlation was observed between AID protein expression and clinicopathological
data of the patients such as age, tumor grade and tumor stage (p>0.05). Our
study is the first one that examines AID protein expression protein in breast cancer tissue
samples in Palestine and in the world. In conclusion, our study showed aberrant expression
of AID protein in Palestinian breast cancer patients, and this study will shed the light on
the cancer research in Palestine.
Description
Keywords
الكيمياء الحيوية والاحياء الجزيئية , Biochemistry & Molecular Biology