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dc.contributor.authorAl-Rimawi, Fuad
dc.contributor.authorHijaz, Faraj
dc.contributor.authorNehela, Yasser
dc.contributor.authorBatuman, Ozgur
dc.contributor.authorKilliny, Nabil
dc.date.accessioned2020-02-23T19:40:35Z
dc.date.available2020-02-23T19:40:35Z
dc.date.issued2019-10-25
dc.identifier.issn2079-6382
dc.identifier.urihttps://dspace.alquds.edu/handle/20.500.12213/5069
dc.description.abstractHuanglongbing (HLB), or citrus greening, is the most destructive disease to the citrus industry. In Florida, it is caused by the bacterium, Candidatus Liberibacter asiaticus (CLas) and is transmitted by the Asian citrus psyllid, Diaphorina citri. Recent studies suggested that antibiotics could inhibit the growth of the CLas pathogen in planta. In the current study, we investigated the uptake and translocation of oxytetracycline and streptomycin in citrus seedlings. Oxytetracycline and streptomycin were delivered via root and stem and their level in various tissues was monitored using enzyme-linked immunosorbent assay (ELISA). Oxytetracycline and streptomycin were detected in the leaves, xylem, phloem, and root after root drench and stem delivery. High levels of antibiotics were detected in the roots after root drench, whereas high levels of antibiotics were detected in the canopy after stem delivery. The level of oxytetracycline detected in the phloem, xylem, and leaves after root drench was higher than that of streptomycin. Whereas the level of streptomycin in root was higher than that of oxytetracycline, indicating that streptomycin was bound to the xylem tissues. Oxytetracycline and streptomycin were detected in the phloem, xylem, leaves, and root tissues thirty-five days after the root incubation in 200 μg.ml-1 solution. These results demonstrated that oxytetracycline and streptomycin were relatively stable and could inhibit CLas growth for a couple of months in citrus trees. Observations reported in this study regarding the distribution and stability of oxytetracycline and streptomycin in citrus plants could be useful for designing an effective program for the control of HLB disease using antibiotics.en_US
dc.description.sponsorshipFunding: National Institute of Food and Agriculture: 2019-70016-29096 Acknowledgments: We thank our lab members for the helpful discussion and technical assistance. This work was generously funded by a grant # 2019-70016-29096 from NIFA-SCRI for OB and NK. Conflicts of Interest: Page: 10 The authors declare no conflict of interesten_US
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.subjectHuanglongbingen_US
dc.subjectoxytetracyclineen_US
dc.subjectstreptomycinen_US
dc.subjectantibioticen_US
dc.subjecttranslocationen_US
dc.subjectcitrusen_US
dc.titleUptake, Translocation, and Stability of Oxytetracycline and Streptomycin in Citrus Plantsen_US
dc.typeArticleen_US


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