Association of G22A polymorphism of the adenosine deaminase (ADA) gene with biochemical characteristics in type 2 diabetic Palestinians
Date
2018-10-31
Authors
Ereqat, S.
Qrea, L.
Nasereddin, A.
Journal Title
Journal ISSN
Volume Title
Publisher
FUNEPC-RP, Ribeirao Preto, SP (Brazil)
Abstract
The adenosine deaminase G22A polymorphism
(20q.11.33) affects the level of adenosine deaminase (ADA) expression,
which plays an important role in the regulation of intracellular and
extracellular concentrations of adenosine. Recent studies reported
greater ADA activity in diabetic patients and showed the role of ADA in
the modulation of insulin activity and glucose homeostasis. We
investigated whether the G22A polymorphism of the ADA gene is
associated with type 2 diabetes mellitus (T2DM) in the Palestinian
population and assessed the relationship between the G22A variant and
fasting plasma glucose (FPG), glycated hemoglobin (HbA1c) and lipid
profile among T2DM patients. A total of 231 individuals with T2DM
and a control sample of 101 non-diabetic participants were randomly
selected from those who were attending United Nations Relief and
Works Agency (UNRWA) clinics for treatment and/or follow up.
Genomic DNA was extracted from peripheral blood samples and PCRRFLP
was performed to identify the TaqI polymorphism G22A of the
ADA gene. No significant differences were observed in the genotype and
allele frequencies between T2DM patients and the control group. Yet, among diabetic patients, the GG genotype was significantly associated
with higher FPG and HbA1c when compared to the GA+AA genotype
but had no influence on blood pressure, BMI or other metabolic
parameters. In conclusion, we confirm that the GG genotype of the ADA
gene is associated with poor glycemic control in T2DM Palestinians and
points to the association of the G22A variant with decreased activity of
the ADA enzyme, which is of paramount importance in the
pathophysiology of T2DM.
Description
Keywords
Diabetes Mellitus Type 2 , Fasting Plasma glucose , Adenosine Deaminase , Glycated Hemoglobin , Palestine
Citation
TY - JOUR AU - Ereqat, Suheir AU - Qrea, lina AU - Nasereddin, Abedelmajeed PY - 2019/06/13 SP - T1 - Association of G22A polymorphism of the adenosine deaminase (ADA) gene with biochemical characteristics in type 2 diabetic Palestinians VL - JO - ER -